Dynamic gene expression changes during sleep in Drosophila brain


Gene expression in the brain of Drosophila melanogaster was measured using GeneChip Drosophila microarrays (Affymetrix) after different durations of sleep and periods of sleep deprivation. There were a total of ten time groups, with three biological replicates. Seven time groups were used to determine differential gene expression between normal and sleep-deprived flies, whereas three time groups were used to test for differential gene expression due to the effect of mechanical stimulation. For further details, see Zimmerman et al., 2006.


README file: README_zimmerman2006.txt
Design file: design.SleepInFlies.dat
CEL files: GEO
Processed data: FlySleep.rma.dat


Zimmerman JE, Rizzo W, Shockley KR, Raizen DM, Naidoo N, Mackiewicz M, Churchill GA, Pack AI
Physiol Genomics. 2006 Nov 27;27(3):337-50 [ Full Text ] PMCID: PMC

Saltwater marsh in Acadia National Park